Aequorin bioluminescence assays on MicroBeta2 LumiJET for studying Ca2+-coupled GPCRs and ion channels
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چکیده
Reconstitution of an active aequorin, using native coelenterazine or its derivative coelenterazine h, yields an enzyme having a fast luminescent response to increasing calcium concentrations, and a high level of signal intensity. The aequorin photoprotein undergoes a bioluminescent reaction in the presence of calcium ions, producing a flash of light peaking at 469 nm. This wavelength correlates well with the maximum quantum efficiency of the PMTs used in the MicroBeta2®. Aequorin bioluminescence assays on MicroBeta2 LumiJET for studying Ca2+-coupled GPCRs and ion channels A P P L I C A T I O N
منابع مشابه
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تاریخ انتشار 2009